An upstream signal is required for in vitro transcription ofNeurospora 5S RNA genes

Abstract
The DNA sequences upstream of the 5S RNA genes inNeurospora crassa are largely different from one another, but share a short consensus sequence located in the segment 29 to 26 nucleotides preceding the transcribed region. Differences among flanking sequences do not appear to affect transcription. Deletion analysis indicates, however, that a DNA segment including the conserved “TATA box” is required for in vitro transcription of Neurospora 5S RNA genes.