Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements
- 1 December 1981
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 294 (5842) , 626-631
- https://doi.org/10.1038/294626a0
Abstract
The split promoter sequences of a .**GRAPHIC**. gene of Xenopus laevis were mapped to nucleotides 13-20 and 51-64 of the tRNALeu coding sequences. The sequences closely coincide with 2 conserved sequence blocks present in all eukaryotic tRNA genes. The 2 conserved sequence blocks were found to be exchangeable between tRNA genes as chimeric tRNAMet -tRNALeu genes proved transcriptionally active. Two prokaryotic tRNA genes exhibiting strong homologies with the 2 blocks yielded specific transcripts when tested in an eukaryotic transcriptional system.Keywords
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