Optimum and Limiting Temperatures for the Growth of the Plague Bacillus in Broth

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Abstract
This is a study of the growth of Pasteurella pestis in 2 lots of nutrient broth, one adjusted at a pH of 6.4 and the other at a pH of 7.2, at various temps. To determine the growth of the cultures at pH 6.4, 8 parallel plates were used for counting the number of organisms in each test tube, and 4-6 detns. were made at each of the temps. 23[degree], 25[degree], 27[degree], 29[degree], 30[degree], and 31 [degree]C. To determine the growth at pH 7.2, 4 sets of detns. with colony counts on 8 parallel plates were made for each of the temps. 25[degree], 27[degree], 28[degree], 29[degree], 30[degree], and 32[degree]C. The growth was greatest at 27[degree]-28[degree]. The lowest and highest temps. at which growth stopped were[long dash]2[degree] and 45[degree], respectively, as detd. by supplementary tests.

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