COMPARISON OF PRODUCTS OF REACTION OF 7-METHYLBENZ-[A]ANTHRACENE 5,6-OXIDE AND RNA, WITH THOSE FORMED IN 7-METHYLBENZ[A]ANTHRACENE-TREATED CELLS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 36  (7) , 2306-2311
Abstract
RNA was isolated by a phenol extraction method from mouse embryo cells treated in culture with [G-3H]-7-methylbenz[a]anthracene or [G-3H]-7-methylbenz[a]anthracene-5,6-oxide (the K-region epoxide). The RNA was degraded to ribonucleosides, mixed with UV-absorbing quantities of the epoxide ribonucleoside products isolated from RNA that reacted with 7-methylbenz[a]anthracene-5,6-oxide in aqueous ethanol solution and chromatographed on a column of Sephadex LH-20 eluted with a methanol:water gradient. The 7-methylbenz[a]anthracene-5,6-oxide ribonucleoside products formed in cells were identical to those formed in aqueous solution, although the relative amounts of the products varied. The majority of these epoxide-ribonucleoside products were not identical to the products formed in cells treated with the parent hydrocarbon. These results suggest that the major reactive form of 7-methylbenz[a]anthracene that binds to RNA in mouse embryo cells is not the K-region epoxide of this hydrocarbon.

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