Stimulation of RNA Transcription from Pea and Corn DNA by Protein Retained on Sepharose Coupled to 2,4-Dichlorophenoxyacetic Acid
- 1 August 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (8) , 1824-1827
- https://doi.org/10.1073/pnas.68.8.1824
Abstract
When crude extracts from pea or corn shoots are passed through a column of agarose coupled to a derivative of the synthetic plant hormone 2,4-dichlorophenoxyacetic acid, certain protein fractions are retained, whereas unmodified agarose adsorbs no protein. Appropriate elution yields a protein factor that stimulates DNA-dependent RNA synthesis (supported by Escherichia coli polymerase) by 40-200%. Preliminary evidence suggests that the factor may be influencing initiation of RNA chains.Keywords
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