Genetic polymorphism of mouse immunoglobulin light chains revealed by isoelectric focusing.

Open Access
Publisher Website
Google Scholar
Abstract
L chains isolated from normal immunoglobulin of unimmunized mice were analyzed by gel isoelectric focusing. Examination of the focusing patterns of L chains from 9 inbred mouse strains showed that 6 of the strains (SWR/J, C3H/HeJ, DBA/1J, A/J, CBA/J and C57BL/6J) possessed a virtually identical spectrum of focusing bands, while the remaining 3 strains (RF/J, AKR/J and C58/J) showed clear differences involving several bands. Analysis of the L chains of individual SWR/J, C58/J and F1 hybrid mice indicated that the differences in focusing pattern were inherited in a simple codominant fashion. A new procedure was developed for the rapid analysis of L chains from small quantities of serum.