Nucleotide sequence of the virG locus of the Agrobacterium tumefaciens plasmid pTiC58
- 1 July 1987
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 1 (1) , 309-316
- https://doi.org/10.1111/j.1365-2958.1987.tb01937.x
Abstract
The nucleotide sequence of the virG locus of the nopaline type plasmid pTiC58 of Agrobacterium tumefaciens has been determined. It contains an open reading frame (ORF) of 759 nucleotides and has 77% homology to the virG sequences of octopine type plasmids. Differences between the sequences of the two types of Ti plasmids in the region of virG are located predominantly outside the ORF. The amino acid sequences inferred from the two virG genes show 80% homology to each other and each shows the same moderate homologies to amino acid sequences derived from genes in a family of two-component regulatory systems. Specific differences in nucleotide and amino acid sequences as well as a structure-function model for the gene product are discussed.Keywords
This publication has 35 references indexed in Scilit:
- The virD operon of Agrobacterium tumefaciens encodes a site-specific endonucleaseCell, 1986
- Generation of single-stranded T-DNA molecules during the initial stages of T-DNA transfer from Agrobacterium tumefaciens to plant cellsNature, 1986
- virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciensCell, 1986
- Cloning and expression of multiple protein kinase C cDNAsCell, 1986
- Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciensNature, 1985
- Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNADNA, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisGene, 1983
- Homology among DNA-binding proteins suggests use of a conserved super-secondary structureNature, 1982
- Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciensJournal of Molecular Biology, 1981