Quantitative Analysis of Photosynthate Unloading in Developing Seeds of Phaseolus vulgaris L.
- 1 June 1992
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 99 (2) , 635-642
- https://doi.org/10.1104/pp.99.2.635
Abstract
The pathway and kinetics of photosynthate unloading in developing seeds of bean (Phaseolus vulgaris L.) were investigated using steady-state labeling with 14CO2. The continuous assimilation of 14CO2 at constant specific activity produced stable tracer fluxes that facilitated straightforward analyses of photosynthate import and unloading in developing seeds. The kinetics of tracer equilibration within intact seeds were compatible with a symplastic route of photosynthate unloading in the seed coat. The import and partitioning of tracer within seeds were partially disrupted by the surgical excision of the distal halves of seeds as practiced during the preparation of “empty” seed coats for perfusion.Keywords
This publication has 12 references indexed in Scilit:
- Phloem Loading in Coleus blumei in the Absence of Carrier-Mediated Uptake of Export Sugar from the ApoplastPlant Physiology, 1990
- Carbon Partitioning among Leaves, Fruits, and Seeds during Development of Phaseolus vulgaris L.Plant Physiology, 1989
- Mild Water Stress of Phaseolus vulgaris Plants Leads to Reduced Starch Synthesis and Extractable Sucrose Phosphate Synthase ActivityPlant Physiology, 1989
- Photosynthesis, Carbohydrate Metabolism, and Export in Beta vulgaris L. and Phaseolus vulgaris L. during Square and Sinusoidal Light RegimesPlant Physiology, 1989
- Seed Growth Rate and Carbohydrate Pool Sizes of the Soybean FruitPlant Physiology, 1985
- Sucrose Concentration at the Apoplastic Interface between Seed Coat and Cotyledons of Developing Soybean SeedsPlant Physiology, 1985
- Sources of Sucrose Translocated from Illuminated Sugar Beet Source LeavesPlant Physiology, 1983
- An In Vivo Technique for the Study of Phloem Unloading in Seed Coats of Developing Soybean SeedsPlant Physiology, 1983
- Kinetics of C-14 Translocation in SoybeanPlant Physiology, 1970
- Kinetics of C-14 Translocation in SoybeanPlant Physiology, 1970