An In Vivo Technique for the Study of Phloem Unloading in Seed Coats of Developing Soybean Seeds
Open Access
- 1 May 1983
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 72 (1) , 268-271
- https://doi.org/10.1104/pp.72.1.268
Abstract
A technique has been developed which permits mechanistic studies of phloem unloading in developing seeds of soybean (Glycine max cv Clark) and other legumes. An opening is cut in the pod wall and the embryo surgically removed from the seedcoat without diminishing the capacity of that tissue for assimilate import, phloem unloading, or efflux. The sites of phloem unloading were accessible via the seedcoat apoplast and were challenged with inhibitors, solutes, buffers, etc., to characterize the unloading process.Keywords
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