PRODUCTION OF L-(1-C-11]VALINE BY HPLC RESOLUTION
- 1 January 1982
- journal article
- research article
- Vol. 23 (1) , 29-33
Abstract
Based on a recently developed analytical technique, preparative high-performance liquid chromatographic (HPLC) resolution of DL-[1-11C]valine [a potential pancreatic imaging agent] was achieved. A conventional reverse-phase HPLC column and a chiral mobile phase (aqueous solution of L-proline, cupric acetate and sodium acetate) were used. The Cu can be removed from the L-valine fraction by precipitation as the sulfide, and final purification by cation-exchange chromatography yields L-[1-11C]valine in a form acceptable for clinical positron tomographic studies. This purification method does not remove the L-proline introduced in the resolution process, but added L-proline did not affect the tissue distribution of L-[1-14C]valine in rats. Up to 60 mCi of L-[1-11C]valine was produced in an overall synthesis and resolution time of 50 min. This procedure adapts to the rapid resolution of other C-11-labeled amino acid racemates.This publication has 8 references indexed in Scilit:
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- Preparation and preliminary tissue studies of optically active 11C-d- and l-phenylalanineThe International Journal of Applied Radiation and Isotopes, 1981
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