Rapid isolation of yeast nuclei
- 1 November 1981
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 4 (2) , 85-90
- https://doi.org/10.1007/bf00365686
Abstract
A procedure has been developed for the rapid isolation of yeast nuclei in high yield using Percoll gradients. The nuclei are substantially free of cytoplasmic contamination as measured by alcohol dehydrogenase activities, have the typical chromatin digestion pattern when digested with nucleases, are useful for isolation of nuclear proteins and for in vitro transcription experiments.Keywords
This publication has 38 references indexed in Scilit:
- Isolation of intact chloroplasts from spinach leaf by centrifugation in gradients of the modified silica "Percoll".Agricultural and Biological Chemistry, 1979
- Rapid isolation of pancreatic islets from collagenase digested pancreas by sedimentation through percoll™ at unit gravityBiochemical and Biophysical Research Communications, 1977
- Quantitative analysis of the digestion of yeast chromatin by staphylococcal nucleaseBiochemistry, 1977
- Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.Proceedings of the National Academy of Sciences, 1977
- Yeast Chromatin Subunit StructureScience, 1975
- Yeast ChromatinEuropean Journal of Biochemistry, 1973
- Density separation of mouse spermatid nucleiExperimental Cell Research, 1972
- ISOLATION OF NUCLEI FROM YEASTThe Journal of cell biology, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Studies on the yeast nucleus: I. The isolation of nucleiBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964