An electrophoretic comparison of enzymes in strains of Gluconobacter species.

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Abstract
Forty-three strains of Gluconobacter spp. were examined for the electrophoretic comparison of 6 enzymes, i.e., glucose-6-phosphate dehydrogenase (NADP-dependent, EC 1.1.1.49), 6-phosphogluconate dehydrogenase (NADP-dependent, EC 1.1.1.44), glucose dehydrogenase (NADP-dependent, EC 1.1.1.119), alcohol dehydrogenase (NAD-dependent, EC 1.1.1.1), aldehyde dehydrogenase (NADP-dependent, EC 1.2.1.4) and catalase (EC 1.11.1.6). From the electrophoretic patterns of the enzymes, these organisms were unequivocally divided into 2 groups, groups I and II. There was no relation between the 2 groups; the similarity value was 0%. Group I consists of organisms with a high guanine-plus-cytosine content of DNA ranging from 58.1-62.8 mol%, a range of 4.7 mol%. Group II is composed of organisms with a distinctly lower guanine-plus-cytosine content of DNA ranging from 54.2-57.6 mol%, a range of 3.4 mol%. Such a grouping was supported earlier by a DNA-DNA hybrid experiment. On the basis of the electrophoretic patterns of the enzymes and the DNA-DNA hybridization, G. cerinus, sp. nov., reviewed name is proposed for the organisms classified in group II.