A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome

Abstract
A physical map for the genome ofSpodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S 1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289–295] was prepared using the restriction endonucleasesSmaI,SfiI,NotI,ApaI,KpnI,BstEII,PstI,SacI, andHindIII. The size of the genome was estimated to 136 kbp for the Morocco isolate (S 1MNPV-M2) and to 135 kbp for the Lyon isolate (S 1MNPV-L4).HindIII-K was used as the zero-point of the map because it hybridized toEcoRI-I andBamHI-F fragment ofAutographa californica (AcMNPV) DNA, and the direction of transcription of theS 1MNPV polyhedrin gene was choosen left to right. Hybridization of sixAcMNPV cloned DNA fragments withS 1MNPV-B genome shows thatS 1MNPV-B andAcMNPV genomic structures are not aligned.