ELECTRON MICROSCOPIC STUDY OF PHAGOCYTOSIS OF STAPHYLOCOCCUS BY HUMAN LEUKOCYTES

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Abstract
Thin sections of leukocytes phagocytizing bacteria (Staphylococcus aureus) were prepared and examined with the electron microscope. The leukocytes and a virulent hemolytic strain of staphylococcus were mixed in vitro and samples removed at intervals up to one hour. Sections from the 5 minutes incubation period showed stages of the actual process of ingestion of the bacteria. The longer incubation times showed the multiplication of bacteria within the neutrophilis and their destruction. Very thin sections showed some bacterial morphology at magnifications of 60,000.