B lymphocyte-specific, Cre-mediated mutagenesis in mice
Open Access
- 1 March 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (6) , 1317-1318
- https://doi.org/10.1093/nar/25.6.1317
Abstract
Adaptation of the P1 phage-derived Cre/loxP site-specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a /oxP-flanked substrate, we find a deletion efficiency of 75–80% in bone marrow-derived pre-B cells that increases to 90–95% in splenic B cells.Keywords
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