Effects of storage conditionsin vitroon the assay of chick liver glycogen

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Abstract
1. The reproducibility of an enzymic method for assaying chick liver glycogen was found to be ± 1.6%. 2. Storage of liver at either ‐21 or ‐70 °C for up to 4 weeks, after freezing in liquid nitrogen, had no significant effect on glycogen concentration. However, the concentration of free glucose increased by about 50% during this time. 3. Storage of liver in ice‐cold saline for up to 3 h proved unsatisfactory because of a significant loss of glycogen. 4. There was no significant difference in immediate glycogen concentration in liver tissue that was freeze‐clamped, frozen in liquid nitrogen or collected into ice‐cold saline. The free glucose concentration was higher, when estimated immediately after, with the latter two methods of collection of liver tissue.