AMINO-ACID-SEQUENCE AT THE ATP-BINDING SITE OF CGMP-DEPENDENT PROTEIN-KINASE
- 1 January 1982
- journal article
- research article
- Vol. 257 (2) , 727-733
Abstract
The amino acid sequence at the ATP-binding site on the [bovine lung] cGMP-dependent protein kinase ws determined. For this determination the enzyme was labeled covalently by 5''-p-fluorosulfonyl[14C]benzoyladenosine and fragmented using cyanogen bromide or digested by trypsin after succinylation. The 14C-labeled peptides were purified by gel filtration and high performance liquid chromatography. When this 37-residue sequence was compared with that of the ATP-binding site of the catalytic subunit of cAMP-dependent protein kinase, a high degree of structural homology was observed for this site in the 2 proteins.This publication has 6 references indexed in Scilit:
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