Effect of Dissolution Conditions of Polyhedral Inclusion Bodies in Sodium Carbonate Solution on the Yield of Nuclear Polyhedrosis Virus of the Silkworm, Bombyx mori (Lepidoptera : Bombycidae)

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Abstract
Polyhedral inclusion bodies (PIBs) of nuclear polyhedrisis virus (NPV) of the silkworm, Bombyx mori, were dissolved in Na2CO3 under various conditions, and yields of virus particles recovered after the centrifugation on sucrose gradients were compared. The yields of virus particles were highly dependent on dissolution temperatures, and at temperatures ranging from 0 to 30°C, they decreased as the dissolution temperatures were elevated. When PIBs were dissolved at low temperatures, concentration of Na2CO3 and time period of Na2CO3 treatments were less crucial for the yields of virus particles. Among media examined for preparation of sucrose gradients, distilled water was most appropriate to obtain a maximal yield of virus particles. These results indicate that alkaline dissolution of PIBs at low temperatures and use of distilled water for preparation of sucrose gradients are essential to produce a maximal yield of virus particles in the purification of B. mori NPV from PIBs.