Myogenic expression of an injectable protease-resistant growth hormone–releasing hormone augments long-term growth in pigs
- 1 December 1999
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 17 (12) , 1179-1183
- https://doi.org/10.1038/70718
Abstract
Ectopic expression of a new serum protease-resistant porcine growth hormone–releasing hormone, directed by an injectable muscle-specific synthetic promoter plasmid vector (pSP-HV-GHRH), elicits growth in pigs. A single 10 mg intramuscular injection of pSP-HV-GHRH DNA followed by electroporation in three-week-old piglets elevated serum GHRH levels by twofold to fourfold, enhanced growth hormone secretion, and increased serum insulin-like growth factor-I by threefold to sixfold over control pigs. After 65 days the average body weight of the pigs injected with pSP-HV-GHRH was ~37% greater than the placebo-injected controls and resulted in a significant reduction in serum urea concentration, indicating a decrease in amino acid catabolism. Evaluation of body composition indicated a uniform increase in mass, with no organomegaly or associated pathology.Keywords
This publication has 23 references indexed in Scilit:
- Growth in Crohn's diseaseActa Paediatrica, 1999
- Enhanced growth by ectopic expression of growth hormone releasing hormone using an injectable myogenic vectorNature Biotechnology, 1997
- GH Treatment Induces Sustained Catch-Up Growth in Children with Intrauterine Growth Retardation: 7-Year ResultsHormone Research, 1997
- Dipeptidyl peptidase IV (DPP-IV) from pig kidney cleaves analogs of bovine growth hormone-releasing factor (bGRF) modified at position 2 with Ser, Thr or Val. Extended DPP-IV substrate specificity?Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1993
- Direct Gene Transfer into Skeletal MuscleIn Vivo: Factors Affecting Efficiency of Transfer and Stability of ExpressionHuman Gene Therapy, 1993
- GRF analogs and fragments: Correlation between receptor binding, activity and structurePeptides, 1991
- In Vitro Stability of Growth Hormone Releasing Factor (GRF) Analogs in Porcine PlasmaHormone and Metabolic Research, 1991
- Modulation of growth hormone (GH) secretion and GH mRNA levels by GH-releasing factor, somatostatin and secretagogues in cultured bovine adenohypophysial cellsJournal of Endocrinology, 1990
- Dipeptidylpeptidase IV and trypsin-like enzymatic degradation of human growth hormone-releasing hormone in plasma.Journal of Clinical Investigation, 1989
- Metabolic clearance and plasma disappearance rates of human pancreatic tumor growth hormone releasing factor in man.Journal of Clinical Investigation, 1984