Action of DNA Polymerase I of Escherichia coli with DNA-RNA Hybrids as Templates
- 1 February 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (2) , 398-402
- https://doi.org/10.1073/pnas.69.2.398
Abstract
Experiments indicating the ability of the ribo strand of a DNA-RNA template to guide polydeoxynucleotide synthesis by highly purified DNA polymerase I of E. coli (EC 2.7.7.7) are presented. With poly(rA)·poly(dT) as template, poly(dT) is formed with a high efficiency, but almost no poly(dA). The specific activity of the enzyme, when tested with this template under suitable conditions, is eight times greater than that found for the poly(dA-dT) template. Single-stranded DNA fractions, with no template activity for DNA polymerase, are converted to efficient templates after their transcription by RNA polymerase. A concerted polymerization reaction, in which the action of DNA polymerase is dependent on that of RNA polymerase, can also be demonstrated with synthetic polydeoxynucleotides and single-stranded fractions of denatured DNA as templates.Keywords
This publication has 12 references indexed in Scilit:
- A Possible Role for RNA Polymerase in the Initiation of M13 DNA SynthesisProceedings of the National Academy of Sciences, 1971
- RNA-Dependent DNA Polymerase: Possible Role in the Amplification of Ribosomal DNA in Xenopus oocytesProceedings of the National Academy of Sciences, 1971
- Synthesis of Amplified DNA That Codes for Ribosomal RNAProceedings of the National Academy of Sciences, 1971
- Template Properties of Complementary Fractions of Denatured Microbial Deoxyribonucleic AcidsProceedings of the National Academy of Sciences, 1970
- Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase.1969
- SEPARATION OF MICROBIAL DEOXYRIBONUCLEIC ACIDS INTO COMPLEMENTARY STRANDSProceedings of the National Academy of Sciences, 1969
- Separation of B. subtilis DNA into complementary strands, I. Biological properties.Proceedings of the National Academy of Sciences, 1968
- Studies on the loss and the restoration of the transforming activity of the deoxyribonucleic acid of Bacillus subtilisBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- The Synthesis of a Deoxyribonucleic Acid Polymer of Alternating Base Sequence from a Deoxyribonucleic Acid-Ribonucleic Acid Hybrid TemplateJournal of Biological Chemistry, 1966
- Comparative properties of DNA, RNA, and hybrid homopolymer pairs.1965