Template Properties of Complementary Fractions of Denatured Microbial Deoxyribonucleic Acids
- 1 April 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 65 (4) , 1049-1056
- https://doi.org/10.1073/pnas.65.4.1049
Abstract
DNA preparations from seven bacterial species and from E. coli phage T4, and also the complementary L and H fractions into which these DNA specimens, after denaturation, were separated by chromatography on methylated albumin-kieselguhr columns, were studied as templates in the RNA polymerase system, and the nucleotide composition of the RNA products was determined. The RNA transcripts of the separated L and H fractions were found to be faithful copies of the respective DNA fractions. This suggests "transcription analysis" as a sensitive and reliable analytical technique for the determination of the base composition of denatured DNA. The L and H fractions of T4 DNA were shown, both by temperature-absorbance profiles and by transcription analysis, to be mutually complementary. The RNA products formed with intact DNA as the template were not exact copies of the latter; their composition indicated that under our experimental conditions the "heavy" DNA strand is transcribed preferentially.Keywords
This publication has 11 references indexed in Scilit:
- SEPARATION OF MICROBIAL DEOXYRIBONUCLEIC ACIDS INTO COMPLEMENTARY STRANDSProceedings of the National Academy of Sciences, 1969
- Seapration of B. subtilis DNA into complementary strands. II. Template functions and composition as determined by transcription with RNA polymerase.Proceedings of the National Academy of Sciences, 1968
- Template functions in the enzymic formation of polyribonucleotides. IV. Denatured DNA as template.Proceedings of the National Academy of Sciences, 1967
- Template functions in the enzymic formation of polyribonucleotides, I. Integrity of the DNA template.Proceedings of the National Academy of Sciences, 1966
- Asymmetric RNA synthesis in vitro: heterologous DNA-enzyme systems; E. coli RNA polymerase.Proceedings of the National Academy of Sciences, 1965
- STRAND SELECTIVE TRANSCRIPTION OF T4 DNA IN VITROProceedings of the National Academy of Sciences, 1964
- Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperatureJournal of Molecular Biology, 1962
- THE ISOLATION OF T4-SPECIFIC RNA ON A DNA-CELLULOSE COLUMNProceedings of the National Academy of Sciences, 1962
- DEOXYRIBONUCLEIC ACID-DIRECTED SYNTHESIS OF RIBONUCLEIC ACID BY AN ENZYME FROM ESCHERICHIA COLIProceedings of the National Academy of Sciences, 1962
- Properties of ribonucleic acid turnover in T2-infected Escherichia coliBiochimica et Biophysica Acta, 1958