STRAND SELECTIVE TRANSCRIPTION OF T4 DNA IN VITRO
- 1 December 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 52 (6) , 1388-1395
- https://doi.org/10.1073/pnas.52.6.1388
Abstract
When fragmented, native T4 DNA served as primer for the E. coli RNA polymerase, over 90 per cent of the synthetic RNA had nucleotide sequences in common with those found in T4 RNA synthesized in vivo. If indeed only one strand of the DNA molecule is transcribed in vivo, then it may be concluded that the same strand was utilized in vitro. However, the strand selection mechanism was destroyed by heat denaturation of the primer T4 DNA, resulting in nearly equal copying of both strands. Sonication of primer T4 DNA also caused a partial loss of strand selectivity.Keywords
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