Activation of yeast plasma membrane ATPase by phorbol ester
- 11 November 1985
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 192 (1) , 95-98
- https://doi.org/10.1016/0014-5793(85)80050-8
Abstract
Addition of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) to yeast cells produces a 2‐fold activation of the plasma membrane ATPase. The activation is reversible and time‐ and dose‐dependent. The activated enzyme shows an increased affinity for its substrate, ATP, and its optimum pH is shifted to a more alkaline range. These changes are similar to those observed in the reported activation by glucose. Upon incubation of yeast cells with 32Pi incorporation of radioactivity in a membrane polypeptide of 105 kDa is observed after addition of either glucose or TPA.Keywords
This publication has 11 references indexed in Scilit:
- Characterization of the activation of Na+/H+ exchange in lymphocytes by phorbol esters: change in cytoplasmic pH dependence of the antiport.Proceedings of the National Academy of Sciences, 1985
- Stimulation of the Na/H exchanger of sea urchin eggs by phorbol esterNature, 1985
- Phorbol ester and diacylglycerol mimic growth factors in raising cytoplasmic pHNature, 1984
- The role of protein kinase C in cell surface signal transduction and tumour promotionNature, 1984
- In vivo glucose activation of the yeast plasma membrane ATPaseFEBS Letters, 1983
- Phosphorylation and Inactivation of Yeast Fructose‐Bisphosphatase in vivo by Glucose and by Proton IonophoresEuropean Journal of Biochemistry, 1982
- Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for proteinAnalytical Biochemistry, 1981
- Reconstitution of the proton-translocating adenosine triphosphatase of yeast plasma membranes.Journal of Biological Chemistry, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- An outer metabolic region of the yeast cellBiochemical Journal, 1950