PURINE AND PYRIMIDINE DERIVATIVES IN MATURE PEA SEEDS

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Abstract
Purine and pyrimidine derivatives in mature pea seeds (Pisum sativum var. Meteor) were identified and determined by methods based on anion-exchange chromatography in a chloride system, elution being by charge adjustment. In contrast with some older reports, neither uric acid nor any other free purine or pyrimidine base could be detected in the seeds. The nucleoside constituents of mature pea seeds were, in descending order of quantitative importance, uridine, adenosine, xanthosine and guanosine. An unusual nucleotide isolated from the seeds was identified as acetyl-3[image]-dephosphocoenzyme A. This was determined both spectrophotometrically and by hydroxamate formation. The crude seed extract showed traces of 2 other hydroxylamine-reacting substances, 1 of which gave rise to succinohydroxamate and the other to malonohydroxamate. Mature seeds of Lupinus luteus and Phaseolus vulgaris exhibited qualitatively similar chromatographic patterns to that of pea seeds. The relationship of the various substances isolated to the metabolic state of dry mature seeds is discussed.