Carbon Nanotube Amplification Strategies for Highly Sensitive Immunodetection of Cancer Biomarkers
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- 1 August 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (34) , 11199-11205
- https://doi.org/10.1021/ja062117e
Abstract
We describe herein the combination of electrochemical immunosensors using single-wall carbon nanotube (SWNT) forest platforms with multi-label secondary antibody-nanotube bioconjugates for highly sensitive detection of a cancer biomarker in serum and tissue lysates. Greatly amplified sensitivity was attained by using bioconjugates featuring horseradish peroxidase (HRP) labels and secondary antibodies (Ab2) linked to carbon nanotubes (CNT) at high HRP/Ab2 ratio. This approach provided a detection limit of 4 pg mL-1 (100 amol mL-1), for prostate specific antigen (PSA) in 10 μL of undiluted calf serum, a mass detection limit of 40 fg. Accurate detection of PSA in human serum samples was demonstrated by comparison to standard ELISA assays. PSA was quantitatively measured in prostate tissue samples for which PSA could not be differentiated by the gold standard immunohistochemical staining method. These easily fabricated SWNT immunosensors show excellent promise for clinical screening of cancer biomarkers and point-of-care diagnostics.Keywords
This publication has 59 references indexed in Scilit:
- Efficacy of infliximab on MRI-determined bone oedema in psoriatic arthritisAnnals of the Rheumatic Diseases, 2007
- Reliability and sensitivity to change of the OMERACT rheumatoid arthritis magnetic resonance imaging score in a multireader, longitudinal settingArthritis & Rheumatism, 2005
- Involvement of subchondral bone marrow in rheumatoid arthritis: Lymphoid neogenesis and in situ relationship to subchondral bone marrow osteoclast recruitmentArthritis & Rheumatism, 2005
- Histomathematical Analysis of Clinical Specimens: Challenges and ProgressJournal of Histochemistry & Cytochemistry, 2005
- Mediated amperometric immunosensing using single walled carbon nanotube forestsThe Analyst, 2004
- Systems Biology: A Brief OverviewScience, 2002
- Sensitive Immunoassay of Tissue Cell Proteins Procured by Laser Capture MicrodissectionThe American Journal of Pathology, 2000
- Structure of the p53 Tumor Suppressor Bound to the Ankyrin and SH3 Domains of 53BP2Science, 1996
- Some Analytical Considerations on the Measurement of Prostate-Specific Antigencclm, 1996
- The american rheumatism association 1987 revised criteria for the classification of rheumatoid arthritisArthritis & Rheumatism, 1988