Differential internalisation of mGluR1 splice variants in response to agonist and phorbol esters in permanently transfected BHK cells
Open Access
- 24 November 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 418 (1-2) , 83-86
- https://doi.org/10.1016/s0014-5793(97)01353-7
Abstract
The internalisation of metabotropic glutamate receptor (mGluR1α) and its splice variant (mGluR1β), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1α and mGluR1β exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma membrane to endosome‐enriched membrane fractions. Agonist challenge however caused a rapid loss, within 5–10 min, of mGluR1β but not mGluR1α from the cell surface. These results show that the two forms of mGluR1 show different internalisation responses to agonist and suggest that the C‐terminal region of the molecule plays an important role in this phenomenon.Keywords
This publication has 25 references indexed in Scilit:
- G-protein-coupled receptor regulation: role of G-protein-coupled receptor kinases and arrestinsCanadian Journal of Physiology and Pharmacology, 1996
- Immunological identification of A1 adenosine receptors in brain cortexJournal of Neuroscience Research, 1995
- Rapid agonist mediated phosphorylation of the metabotropic glutamate receptor 1α by protein kinase C in permanently transfected BHK cellsFEBS Letters, 1995
- The metabotropic glutamate receptors: Structure and functionsNeuropharmacology, 1995
- Differential cellular localization of three splice variants of the mGluR1 metabotropic glutamate receptor in rat cerebellumNeuroReport, 1994
- A Comparison of Two Alternatively Spliced Forms of a Metabotropic Glutamate Receptor Coupled to Phosphoinositide TurnoverJournal of Neurochemistry, 1993
- A family of metabotropic glutamate receptorsNeuron, 1992
- A sensitive protein assay method using micro-titer platesCellular and Molecular Life Sciences, 1986
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970