Rapid agonist mediated phosphorylation of the metabotropic glutamate receptor 1α by protein kinase C in permanently transfected BHK cells
Open Access
- 3 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 367 (3) , 301-305
- https://doi.org/10.1016/0014-5793(95)00575-t
Abstract
Clonal BHK cells permanently transfected with the metabotropic glutamate receptor 1α (mGluR1α), which is coupled to phospholipase C, were used to study the phosphorylation state of the receptor. Cells were labelled with 32PO4 3−, lysed, the receptor immunoprecipitated with specific anti‐peptide antibodies and the immunoprecipitates analysed by SDS‐PAGE followed by autoradiography. A significant basal level of receptor phosphorylation was observed which was rapidly and transiently increased in response to agonist activation of the receptor. This agonist effect was found to be dose dependent with a rapid time course and could be abolished by the specific PKC inhibitor Ro318220, suggesting that PKC was responsible for the agonist mediated phosphorylation of the receptor.Keywords
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