Purification, structure determination and synthesis of covalitoxin‐II, a short insect‐specific neurotoxic peptide from the venom of the Coremiocnemis validus (Singapore tarantula)
Open Access
- 26 May 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 474 (2-3) , 208-212
- https://doi.org/10.1016/s0014-5793(00)01593-3
Abstract
Spider venoms contain toxins that specifically immobilize and kill insects. We report the purification and characterization of a new insect‐specific toxin named covalitoxin‐II (Cvtx‐II; mass, 3406.24±0.64), from Coremiocnemis validus (Singapore tarantula) venom. The complete 31 amino acid sequence of Cvtx‐II has been determined and it shows less than 40% identity with spider toxins. However, Cvtx‐II has conserved cystine motif analogous to other spider and ω‐conotoxins. Cvtx‐II was chemically synthesized and identified with the native Cvtx‐II. Synthetic Cvtx‐II induced insect‐specific non‐lethal excitatory activity when injected into crickets, but not in cockroaches and mice.Keywords
This publication has 43 references indexed in Scilit:
- Binding of Ala‐scanning analogs of ω‐conotoxin MVIIC to N‐ and P/Q‐type calcium channelsFEBS Letters, 2000
- Combinatorial synthesis of ω‐conotoxin MVIIC analogues and their binding with N‐ and P/Q‐type calcium channelsFEBS Letters, 2000
- Synthesis, Bioactivity, and Cloning of the L-Type Calcium Channel Blocker ω-Conotoxin TxVIIBiochemistry, 1999
- Binding of chimeric analogs of ω‐conotoxin MVIIA and MVIIC to the N‐ and P/Q‐type calcium channelsFEBS Letters, 1997
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Characterization and cloning of insecticidal peptides from the primitive weaving spider Diguetia canitiesInsect Biochemistry and Molecular Biology, 1995
- An inhibitor of the Kv2.1 potassium channel isolated from the venom of a Chilean tarantulaNeuron, 1995
- The purification and amino acid sequences of four Tx2 neurotoxins from the venom of the Brazilian ‘armed’ spider Phoneutria nigriventer (Keys)FEBS Letters, 1992
- Primary structure of scorpion anti‐insect toxins isolated from the venom of Leiurus quinquestriatus quinquestriatusFEBS Letters, 1990
- Neurotoxins active on insects: amino acid sequences, chemical modifications, and secondary structure estimation by circular dichroism of toxins from the scorpion Androctonus australis HectorBiochemistry, 1990