Membrane filtration differentiation ofE. colifrom coliforms in the examination of water
- 1 September 1989
- journal article
- research article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 67 (3) , 343-346
- https://doi.org/10.1111/j.1365-2672.1989.tb02503.x
Abstract
A membrane filter-Endo agar method for enumerating Escherichia coli as distinct from other coliforms in drinking water was developed. Membranes containing coliform colonies are transferred to nutrient agar containing 4-methyl umbelliferyl-.beta.-D-glucuronide (MUG) and incubated at 3.degree. C for 4 h. The MUG is hydrolyzed by the glucuronidase of E. coli and the fluorogenic product is visualized. The method recovered 98% of E. coli without false positives and is proposed as an additional test in routine water examination for the detection of pollution.This publication has 22 references indexed in Scilit:
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