Patterns of gene expression in rat bone marrow stromal cells cultured on titanium alloy discs of different roughness

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Abstract
Rat bone marrow stromal cells were cultured on either Ra (0.14 μm) or Ra (5.8 μm) Ti6Al4V discs for 24 or 48 h. Cells on the Ra (0.14 μm) surface showed typical fibroblastic morphology, whereas cells on the Ra (5.8 μm) surface were in clusters with a more epithelial appearance. RNA was extracted from the cells at both time points, and gene expression was analyzed by using a rat gene microarray. At 24 and 48 h, a similar number of genes were both up- and down-regulated at least twofold on the Ra (5.8 μm) surface compared to the Ra (0.14 μm) surface. We analyzed the relative level of specific groups of genes related to bone and cartilage development, cell adhesion and extracellular matrix proteins, transcription factors, bone morphogenetic proteins, phospholipases, and protein kinases. Roughness did not appear to be a specific stimulator of osteogenesis because genes of both the bone and cartilage lineage were up-regulated on the Ra (5.8 μm) surface. The most prominent change among transcription factors was up-regulation of Hox 1.4 on the Ra (5.8 μm) surface. Up-regulation of phospholipase A2 and SMAD 4 indicate these genes are also involved in the response of cells to an Ra (5.8 μm) surface. Our data show surface roughness alters the expression of a large number of genes in marrow stromal cells, which are related to multiple pathways of mesenchymal cell differentiation. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 391–401, 2004

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