An Improvement in Quantitative Separation of Phospholipids with Silicic Acid Impregnated Filter Paper

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Abstract
A technique is described for avoiding loss of lecithin at the point of origin during quantitative separation of phospholipids. Application of lipid extracts was made on an unimpregnated area of the filter paper before starting the solvent run giving the lecithin a "running start" and avoiding fixation to the silicic acid. Recoveries of lecithin improved 7-10%. Recoveries of sphingomyelin and lysolecithin were unaffected, their being little binding at the origin with the usual procedure.

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