Isolation and characterization of Schizosaccharomyces pombe mutants with defective NAD-dependent malic enzyme
- 1 June 1986
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 32 (6) , 481-486
- https://doi.org/10.1139/m86-088
Abstract
To obtain NAD-dependent malic enzyme mutants of Schizosaccharomyces pombe, a colony color indicator screening system was developed. Mutants defective for malic acid utilization (mau mutants) are yellow, while wild-type colonies are blue on the defined bromcresol green based indicator medium. NAD-dependent malic enzyme mutants were distinguished from other mau mutants by subsequent, starch gel electrophoresis, spectrophotometry, complementation tests, and intermediate pool analysis with cell-free extracts.This publication has 11 references indexed in Scilit:
- A revised chromosome map of the fission yeast Schizosaccharomyces pombeCurrent Genetics, 1984
- Cloning the Gene for the Malolactic Fermentation of Wine from Lactobacillus delbrueckii in Escherichia coli and YeastsApplied and Environmental Microbiology, 1984
- The anaerobic metabolism of malate of Saccharomyces bailii and the partial purification and characterization of malic enzymeArchiv für Mikrobiologie, 1982
- REGULATORY GENES CONTROLLING MITOSIS IN THE FISSION YEAST SCHIZOSACCHAROMYCES POMBEGenetics, 1980
- Identification and biochemical analysis of mouse mutants deficient in cytoplasmic malic enzymeBiochemistry, 1980
- GENETIC MAPPING IN SCHIZOSACCHAROMYCES POMBE BY MITOTIC AND MEIOTIC ANALYSIS AND INDUCED HAPLOIDIZATIONGenetics, 1977
- Regulation and Function of Pyruvate Kinase and Malate Enzyme in Yeast*European Journal of Biochemistry, 1967
- CATABOLITE REPRESSION OF MALIC DEHYDROGENASE IN NEUROSPORA CRASSACanadian Journal of Microbiology, 1967
- Screening of auxotrophic mutants of schizosaccharomyces pombe with 2-deoxyglucoseMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1965
- The metabolism of l-malate and other compounds by schizosaccharomyces pombeArchiv für Mikrobiologie, 1963