Identification of disulfide bonds in the ninth component (C9) of human complement
Open Access
- 12 February 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 380 (1-2) , 8-12
- https://doi.org/10.1016/0014-5793(95)01541-8
Abstract
C9 is the most abundant protein of the membrane attack complex of complement. By means of limited proteolysis, different chromatographic techniques, a thiol‐specific fluorescence assay, amino acid analysis, and Edman degradation, 9 out of 12 disulfide bridges are definitely assigned (Cys22Cys57, Cys33Cys36, Cys67Cys73, Cys121Cys160, Cys233Cys234, Cys359Cys384, Cys489Cys505, Cys492Cys507, Cys509Cys518). Weaker evidence permits to reduce the number of possible configurations for the remaining 3 cystines (Cys80Cys91, Cys86Cys104, Cys98Cys113, or Cys80Cys91, Cys86Cys113, Cys98Cys104). These findings are discussed in comparison with the strongly related components C6, C7, C8α, and C8β.Keywords
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