Factors Affecting the Production of Amylase by Aspergillus niger , Strain NRRL337, When Grown in Submerged Culture

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Abstract
Laboratory and pilot plant fermentations confirm the observations of the Northern Regional Research Laboratory workers on the efficiency of A. niger, strain NRRL337, as a converting agent for grain fermentations. Amylase production by this culture is stimulated by addition of small quantities of NaCl or KCl to the growing medium. For satisfactory alcohol yields, a minimum of 20% conversion by the test described is required when 10% by volume of fungal amylase prepn. is employed in grain fermentations. Yields of from 6-6.3 proof gallons of ethyl alcohol per bushel of wet corn have been obtained in the laboratory, and 5.6 proof gallons of alcohol, on the same basis, have been obtained in pilot plant fermentations using fungal amylase produced as described. Na pentachlorphenate and ammonium bifluoride may be employed to control bacterial contamination in fungal amylase production by A. niger NRRL337. The inhibitive action of Na pentachlorphenate on the sporulation of A. niger in shake flasks has been noted. Sporulation in laboratory shake flasks is frequently correlated with low amylase production, and consequent low alcohol yield in the subsequent yeast fermentation of the converted grain mash.

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