GENETICALLY ENGINEERED SYNTHESIS OF NATURAL PRODUCTS: From Alkaloids to Corrins
- 1 October 1996
- journal article
- review article
- Published by Annual Reviews in Annual Review of Microbiology
- Vol. 50 (1) , 467-490
- https://doi.org/10.1146/annurev.micro.50.1.467
Abstract
▪ Abstract Because many natural products are of biological and medicinal importance, methods are continually being sought for studying their biosynthetic pathways, which may eventually result in increased production and the generation of novel compounds. Advances in genetic engineering have enabled the homologous or heterologous expression of many natural product biosynthetic genes from divergent sources, resulting in a supply of enzymes not readily available by isolation from the producing organism. Mixing and matching of these enzymes in cell-free reactions can provide information, not available by any other means, about enzyme mechanisms, pathway intermediates, and possible variations in the structure of the final product.Keywords
This publication has 65 references indexed in Scilit:
- Reconstitution of apo‐porphobilinogen deaminase: Structural changes induced by cofactor bindingPublished by Wiley ,2001
- Enzymatic synthesis of (6R)- and (6S)-fluoroshikimic acidsBioorganic & Medicinal Chemistry Letters, 1995
- Design of a reusable enzymatic system for the preparation of porphyrins of biological interestBioorganic & Medicinal Chemistry Letters, 1994
- The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthaseFEBS Letters, 1993
- Biosynthesis of vitamin B12FEBS Letters, 1993
- Investigation of the mechanism and steric course of the reaction catalyzed by 6-methylsalicylic acid synthase from Penicillium patulum using (R)-[1-13C;2-2H] and (S)-[1-13C; 2-2H]malonatesBiochemistry, 1992
- Expression of 9 Salmonella typhimurium enzymes for cobinamide synthesis Identification of the 11‐methyl and 20‐methyl transferases of corrin biosynthesisFEBS Letters, 1992
- Enzymatic synthesis of dihydrosirohydrochlorin (precorrin‐2) and of a novel pyrrocorphin by uroporphyrinogen III methylaseFEBS Letters, 1990
- Identification of a cysteine residue as the binding site for the dipyrromethane cofactor at the active site of Escherichia coli porphobilinogen deaminaseFEBS Letters, 1988
- Penicillin biosynthesis: multiple pathways from a modified substrateJournal of the Chemical Society, Chemical Communications, 1984