A microfabricated device for sizing and sorting DNA molecules
Open Access
- 5 January 1999
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 96 (1) , 11-13
- https://doi.org/10.1073/pnas.96.1.11
Abstract
We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2–200 kbp.Keywords
This publication has 11 references indexed in Scilit:
- High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mappingProceedings of the National Academy of Sciences, 1998
- Soft LithographyAngewandte Chemie International Edition in English, 1998
- Self-Sorting of White Blood Cells in a LatticePhysical Review Letters, 1997
- Integrated Capillary Electrophoresis on Flexible Silicone Microdevices: Analysis of DNA Restriction Fragments and Detection of Single DNA Molecules on MicrochipsAnalytical Chemistry, 1997
- Patterned Delivery of Immunoglobulins to Surfaces Using Microfluidic NetworksScience, 1997
- Large DNA fragment sizing by flow cytometry: application to the characterization of P1 artificial chromosome (PAC) clonesNucleic Acids Research, 1996
- A new strategy for genome sequencingNature, 1996
- Fabrication of Submicrometer Features on Curved Substrates by Microcontact PrintingScience, 1995
- Fluorescence detection and size measurement of single DNA moleculesAnalytical Chemistry, 1993
- Sizing single DNA moleculesNature, 1992