Fractionation and Characterization of Arylamidases in Regenerating Wound Tissue.

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Abstract
The formation and characteristics of enzymes hydrolysing particularly 2-naphthylamides of L-valine and L-methionine in the healing wound tissue of the rat were studied. The arylamidase activities were separated from 1 day old wounds into several enzyme fractions by CM and DEAE cellulose chro-matography and by gel filtration on Sephadex G-200. There is more arylamidase activity toward 2-naphthylamides of L-valine and L-methionine in the wound tissue than in the normal skin. The disc electro- phoretic studies showed that new protein bands could be seen and that they coincided with the increased arylamidase activity. In general, the molecular weights of the 1 day old wound tissue arylamidases seemed to be lower than that in the normal skin enzymes as estimated by gel filtration. In the 2 day old wounds, the arylamidases had larger molecular weights than the enzymes 1 day earlier. The effect of cysteine, dithio-threitol, reduced glutathione, EDTA, certain SH-reagents, and heavy metal cations on the action of the arylamidases was studied. The results were compared with those of other investigations on arylamidases of animal tissues.