ESTIMATION OF VIBRIO-CHOLERAE AND ESCHERICHIA-COLI HEAT-LABILE ENTERO-TOXIN BY ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA)

  • 1 January 1980
    • journal article
    • research article
    • Vol. 27  (1) , 89-97
Abstract
By Enzyme-Linked Immunosorbent Assay (ELISA) purified cholera and E. coli enterotoxins can be detected as sensitively as by CHO [Chinese hamster ovary] cells. There is a linear relatinoship between toxin concentrations and extinction values. In plates sensitized with toxins, antitoxins can be titrated with high sensitivity. ELISA cross-titration experiments demonstrate the existing antigenic relationship between cholera toxin and heat labile E. coli enterotoxin. Plates sensitized with anti-E. coli-IgG or anti-cholera-IgG are suitable for detecting cholera toxin and E. coli LT. ELISA seems to be a simple, sensitive and economic method for quantitation of enterotoxins and toxin-specific antibodies.