3‘-Nitrophenylpropyloxycarbonyl (NPPOC) Protecting Groups for High-Fidelity Automated 5‘ → 3‘ Photochemical DNA Synthesis
- 29 March 2001
- journal article
- Published by American Chemical Society (ACS) in Organic Letters
- Vol. 3 (8) , 1105-1108
- https://doi.org/10.1021/ol0069150
Abstract
The most powerful DNA microarrays would be prepared by photolithography with free 3‘-ends that could be processed enzymatically. A photoremovable group that could be removed in quantitative yield would ensure high purity of the synthesized probes. We have developed new pyrimidine building blocks for 5‘ → 3‘ DNA synthesis with high cycle yields using the NPPOC (3‘-nitrophenylpropyloxycarbonyl) protecting group. These phosphoramidites were proved in automated photochemical DNA synthesis on a modified synthesizer.Keywords
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