Characterization of IgG Fc Receptors Induced by Human Cytomegalovirus

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Abstract
Summary Immunoglobulin G Fc receptors (IgG FcRs) induced by human cytomegalovirus (HCMV) were isolated from solubilized HCMV-infected cell extracts by IgG affinity chromatography and analysed by Western blotting using 125I-labelled human IgG and Fc. FcR species of M r 130K, 65K, 50K and 38K were found to mediate the binding of IgG. The 130K and 65K FcRs were also detected on HCMV virions. All four FcRs were reactive with a murine monoclonal antibody to herpes simplex virus glycoprotein E. Anti-HCMV antibodies markedly inhibited the binding of 125I-labelled human IgG Fc to the 130K and 50K species but inhibited to a lesser extent the binding to the 65K FcR.