IGG-FC RECEPTOR INDUCED IN CYTOMEGALOVIRUS-INFECTED HUMAN FIBROBLASTS

  • 1 January 1976
    • journal article
    • research article
    • Vol. 116  (3) , 772-777
Abstract
Cytomegalovirus-(CMV) infected cultured human fibroblasts incubated with normal human serum reacted with Ig[immunoglobulin]G by direct and indirect fluorescent antibody tests. The binding of IgG to infected cells was unrelated to the presence of anti-CMV complement fixing (CF) antibodies and was not observed with uninfected cells. The reaction was first observed 36 h after infection as diffuse cytoplasmic staining, which by 72 h became localized into a dense perinuclear structure. The cytoplasmic fluorescence was not detected with anti-IgA or anti-IgM fluorescent conjugates. The reaction was seen with purified IgG and Fc fragments but was only minimally detectable with Fab fragments. It occurred in fibroblasts infected with 3 standard laboratory strains of CMV and 7 recent CMV isolates from patients, and was observed in 6 separate lots of human foreskin fibroblast cultures as well as in [human lung] WI-38 cells. CMV infection induces the formation of a IgG receptor in human fibroblasts.