Hemadsorption by Herpes Simplex-Infected Cell Cultures

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Abstract
Herpes simplex-infected human cell cultures adsorb sheep erythrocytes sensitized by rabbit anti-sheep erythrocyte antibodies. Still more reliable results of hemadsorption were obtained by a procedure in which infected cell cultures were incubated with the anti-sheep erythrocyte serum and thereafter erythrocytes were added. The capacity to mediate hemadsorption did not depend on the antibody specificity of the antisera, since besides anti-sheep erythrocyte serum, antisera with antiglobulin antibodies gave positive hemadsorption tests. The antibody combining sites do not participate in the reaction with infected cell cultures as determined by experiments which showed that the 5S fragment of rabbit anti-sheep erythrocyte antibody is incapable of mediating hemadsorption. Hemadsorption could be inhibited by rabbit and human antisera to herpes simplex virus but not by antisera to other viruses. Hemadsorption could also be inhibited by rabbit and human antisera with antibodies to cell surface antigens. Virus infection probably produced on the cell surface new sites with affinity to Fc fragment of some antibodies. These sites resemble cytophilic receptor sites.

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