Synthesis of spin-labeled photoaffinity derivatives of NAD+and their interaction with lactate dehydrogenase
- 23 February 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 212 (2) , 203-207
- https://doi.org/10.1016/0014-5793(87)81345-5
Abstract
The synthesis of NAD+ derivatives spin-labeled at either N6 or C8 of the adenine ring is described, in which the carboxamide function of the nicotinamide moiety is replaced by a diazirine ring. Irradiation of these compounds at 350 nm generates a carbene which will react with any functional group in its vicinity including hydrocarbons. Both NAD+ derivatives form tight ternary complexes with lactate dehydrogenase and were covalently incorporated into this enzyme. They may be employed for ESR studies when non-covalent interactions are too weak for motionally restricted species to be observed.Keywords
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