Partial Purification of Viruses with an Anion Exchange Resin.

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Abstract
A simple and rapid procedure for the partial purification of poliomyelitis viruses, utilizing a strong-base anion exchange resin (Amberlite-XE-67, has been developed. The method is applicable to the extraction of the Lansing strain of poliomyelitis virus from CNS-tissue, poliomyelitis virus from human feces and Theiler virus (TO) from mouse feces. With the use of the anion exchange resin, it is possible to adsorb viruses to the resin along with the extraneous nitrogenous material. The virus is then selectively eluted from the resin. A clear, colorless solution is obtained which retains its original infectivity but which has lost at least 90% of its nitrogenous material.
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