The Precipitating Effect of Methanol on Viruses.

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Abstract
Five viruses (Eastern equine encephalomyelitis, MFF human poliomyelitis, mumps, influenza B, and Newcastle disease) were utilized in studying the precipitating effect on them of methanol. Optimal methanol concns. and optimal pH for elution were detd. for each virus. All of the viruses studied demonstrated a relationship between the pH of the elution fluid and solubility: as the pH was raised there was a corresponding increase in solubility of the virus. The methanol precipitation technic results in considerable loss of N from the virus suspension, but this loss does not appear to be at the expense of the virus.