High-speed analysis of multiplexed short tandem repeats with an electrophoretic microdevice

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Abstract
We report the development of a robust and effective method for multiplexed short tandem repeat (STR) analysis within a chip‐based microdevice. The method uses a laser‐induced fluorescence detection system and simultaneously detects three‐ and four‐color multiplexed polymerase chain reaction (PCR) samples. Analyses of the eight combined DNA index system (CODIS) STR loci were performed in 20 min with single‐base‐pair resolution ranging from 0.75 to 1. A simultaneous analysis of fifteen loci‐ladders and a gender marker Amelogenin based on the PowerPlex™ 16 System was achieved in less than 35 min. The system is capable of repetitive operation and may be extended to high‐throughput multilane devices that could be readily interfaced to an automated sample loading system.