Discontinuous electrophoresis of β-caseins for the determination of bovine caseins in milk and dairy products

Publisher Website
Google Scholar
Abstract
Discontinuous acidic anodic polyacrylamide gel electrophoresis enables the separation of bovine β-caseins from those of ovine and caprine. Interfering protein bands as a consequence of ripening or processing have not been detected. After evaluation of the stained gel by laser densitometry, quantification was performed with calibration standards on the same gel by the ratio of the peak areas from bovine to ovine and bovine to caprine, respectively. Thus, independence from the extractability of proteins affected by denaturation and ripening (which might in some cases raise the limit of detection) is achieved. The range of quantification extends from 5 to approximately 70% bovine casein in relation to total casein.