Complete down‐regulation of low‐density lipoprotein receptor activity in human liver parenchymal cells by β‐very‐low‐density lipoprotein
- 5 August 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 287 (1-2) , 34-38
- https://doi.org/10.1016/0014-5793(91)80010-z
Abstract
The effect of LDL and β-VLDL on the expression of the LDL receptor is studied in cultured human parenchymal cells. The high affinity binding of [125I]LDL to cultured human parenchymal cells was down regulated to 37.3±2.9% and 24.0±2.6% of the control value, after preincubation with LDL or β-VLDL for 22 h, respectively. When LDL receptor synthesis was blocked at 22 h a residual receptor activity of 29% is noticed, indicating a half-life of LDL receptors in human parenchymal cells of 12 h. It is concluded that LDL receptor expression on human liver parenchymal cells is subject to complete down-regulation by β-VLDL, which may be held responsible for the cholesterol-rich diet induced down-regulation of LDL receptors, in vivoKeywords
This publication has 28 references indexed in Scilit:
- Comparison of taurocholate accumulation in cultured hepatocytes of pig, rat and manBiochemical and Biophysical Research Communications, 1989
- Lipoprotein binding to cultured human hepatoma cells.Journal of Clinical Investigation, 1987
- Immunoblotting and ligand blotting of the low-density lipoprotein receptor of human liver, HepG2 cells and HeLa cellsFEBS Letters, 1987
- Regulation of low density lipoprotein receptor activity in primary cultures of human hepatocytes by serum lipoproteinsHepatology, 1986
- Ketoconazole blocks bile acid synthesis in hepatocyte monolayer cultures and in vivo in rat by inhibiting cholesterol 7 alpha-hydroxylase.Journal of Clinical Investigation, 1986
- Stimulation of the LDL receptor activity in the human hepatoma cell line Hep G2 by high-density serum fractionsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- High‐affinity association and degradation of 125I‐labelled low density lipoproteins by human hepatocytes in primary cultureFEBS Letters, 1985
- Liver Transplantation to Provide Low-Density-Lipoprotein Receptors and Lower Plasma Cholesterol in a Child with Homozygous Familial HypercholesterolemiaNew England Journal of Medicine, 1984
- A saturable, high-affinity binding site for human low density lipoprotein on freshly isolated rat hepatocytesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- The metabolism of very low density lipoprotein proteins I. Preliminary in vitro and in vivo observationsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1972