Undifferentiated Growth of Orchid Embryos on Media Containing Barbiturates

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Abstract
The effect of Na dodecyl sulfate (SDS) on the gastric secretory response to histamine in dogs was studied. The esophagus and pylorus were tied, a gastric fistula was made, and a catheter placed into the upper third of the duodenum. Histamine dichloride (3 mg.) was injd. subcut. at the end of the operation, 1 hr. later, and every 15 mins. thereafter. Gastric juice was collected for 1/2-hr. periods for 6-9 hrs. The stomach was filled with 2% SDS, a pressure of 4 cm. of water maintained for 30 mins., the stomach was emptied, and samples were collected for 30-min. periods continued for 4 hrs. Water and electrolyte loss was replaced with an isotonic NaCl-HCl mixture through the catheter. A 30 min. contact of SDS with the gastric mucosa caused a striking and prolonged reduction of the gastric secretory response to large doses of histamine. Free acidity was reduced to zero. The secretion decreased for 4 hrs. to approx. 50% of that of a control period. Pepsin concn. was unchanged. Mucin concn. was increased 2.5-5.2 times. Total Cl conc. decreased from 158-160 meq./l. in the control period to 125-430 meq./l. There was no histologic evidence of mucosal irritation after 2 hrs.

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