THE BIOTRANSFORMATION OF ALLYL ALCOHOL AND ACROLEIN IN RAT-LIVER AND LUNG PREPARATIONS

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (5) , 305-308
Abstract
The biotransformation of allyl alcohol and acrolein by rat lung and liver preparations was investigated by measuring acrolein, acrylic acid, glycidol and glycidaldehyde. Acrolein was detected by highpressure liquid chromatography from incubation mixtures containing allyl alcohol, NAD+ and liver 9000 g supernatant fraction or cytosol. Acrolein was not formed when lung fractions were treated similarly. Addition of pyrazole in the incubation mixture inhibited the reaction. The metabolism of acrolein to acrylic acid by liver 9000 g supernatant fraction, cytosol and microsomes was demonstrated; acrylic acid formation was greater with NAD+ than with NADP+ in all 3 fractions. Acrylic acid was formed from allyl alcohol. Disulfiram inhibited the NAD+- and NADP+-dependent reactions. Acrylic acid was not formed when lung preparations were used. Lung and liver microsomal epoxidation products of allyl alcohol and acrolein were identified. Conversion of glycidol to glycerol and glycidaldehyde to glyceraldehyde by liver epoxide hydrase was demonstrated. Epoxides, glycidol and glycidaldehyde were substrates for lung and liver cytosolic glutathione S-transferases.